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Volume 64, Number 6, November-December 2009
Page(s) 383 - 392
DOI 10.1051/fruits:2009034
Published online 08 December 2009

Fruits 64 (2009) 383-392
DOI: 10.1051/fruits:2009034

Nursery hardening of in vitro-produced banana plants

John Charles Robinson1 and Victor Galán Sáuco2

1  Du Roi Lab., P.O. Box 1147, Letsitele 0885, South Africa
2  Inst. Canario Investig. Agrar. (ICIA), Apartado Correos 60, 38208 La Laguna, Tenerife, Canary Islands, Spain

Published online: 8 December 2009

Abstract - Introduction. This protocol can be used as a guide for people who wish to construct their own nursery. It describes the methods for receiving, transplanting, managing and hardening young banana tissue culture plants after the weaning stage. The principle, key advantages, starting plant material and time required are presented. Materials and methods. This part describes the nursery construction, the nursery media, fertiliser and bags, the handling and transplanting of weaned plants into bags (3 steps), and the management schedule for hardening nursery plants (8 steps). Possible troubleshooting is listed. Results. All the characteristics of nursery-hardened plants in bags, ready for transplanting to the field, are formulated.


Résumé - Introduction. Ce protocole peut être utilisé comme un guide pour les personnes qui souhaitent construire leur propre pépinière. Il décrit les méthodes pour recevoir, transplanter, contrôler et endurcir de jeunes bananiers issus de la culture de tissu après sevrage des plants. Le principe, les principaux avantages de la méthode, le matériel végétal de départ et le temps requis sont présentés. Matériel et méthodes. Cette partie décrit la construction de pépinière, les milieux, engrais et sacs utilisés en pépinière, la manipulation et la transplantation des plants sevrés conditionnés en sacs (3 étapes), le programme de gestion pour endurcir les plantes en pépinière (8 étapes). Des problèmes éventuels sont énumérés. Résultats. Toutes les caractéristiques des plantes endurcies en pépinière, conditionnées en sacs et prêtes à être transplantées au champ sont énoncées.


Key words: South Africa / Spain / Musa sp. / methods / vitroplants / hardening

Mots clés : Afrique du Sud / Espagne / Musa sp. / méthode / vitroplant / durcissement

Corresponding author: Victor Galán Sáuco vgalan@icia.es

© CIRAD, EDP Sciences 2009


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