In vitro organogenesis from adult tissue of ‘Bahia’ sweet orange (Citrus sinensis L. Osbeck)

Issue		Fruits Volume 61, Number 6, November-December 2006


Page(s)		367 - 371
DOI		10.1051/fruits:2006036
Published online		23 December 2006

Fruits 61 (2006) 367-371
DOI: 10.1051/fruits:2006036

In vitro organogenesis from adult tissue of 'Bahia' sweet orange (Citrus sinensis L. Osbeck)

Rosely Pereira da Silva^a, Weliton Antonio Bastos de Almeida^b, Elma dos Santos Souza^b and Francisco de Assis Alves Mourão Filho^a

^a Universidade de São Paulo (USP), Escola Superior de Agricultura Luiz de Queiroz (Esalq), Dep. de Produção Vegetal, Caixa Postal 9, CEP 13418-900 Piracicaba, SP, Brasil
^b Universidade Federal da Bahia (UFBA), Faculdade de Agronomia, Dep. de Fitotecnia, CEP 44380-000 Cruz das Almas, BA, Brasil

(Published online 23 December 2006)

Abstract - Introduction. Plant regeneration from in vitro culture of adult citrus tissue may help in early evaluation of important horticultural characteristics of genetic material from breeding programs, which incorporate biotechnological tools. Our research aimed to optimize in vitro culture conditions that could lead to the maximum plant regeneration through organogenesis of 'Bahia' sweet orange (Citrus sinensis L. Osbeck) in explants (internodes and foliar disc segments) collected from adult plants grown under greenhouse conditions. Materials and methods. Organogenesis was induced in internodes on DBA3 culture media supplemented with BAP at (0.0, 1.0, 2.0 and 3.0) mg·L^-1 combined with NAA (0.5 mg·L^-1), and in foliar discs on culture media with BAP at (0.0, 0.5, 1.0, 1.5 and 2.0) mg·L^-1 combined or not with NAA (0.5 mg.L^-1). Results and discussion. The concentrations of (1.0, 2.0 or 3.0) mg·L^-1 of BAP combined with 0.5 mg·L^-1 of NAA induced in vitro organogenesis in internodes of 'Bahia' sweet orange. The use of foliar discs was not efficient for adventitious organogenesis.

Résumé - Introduction. La régénération de plants adultes d'agrumes par culture in vitro de tissus peut aider à l'évaluation précoce d'importantes caractéristiques horticoles de matériel issu de programmes d'amélioration génétique utilisant des outils de biotechnologie. Nos recherches ont cherché à optimiser les conditions de culture in vitro qui pourraient mener à une régénération maximale de plants par organogenèse d'oranger 'Bahia' (C. sinensis L. Osbeck) à partir d'explants (segments d'entre-noeuds et disques foliaires) prélevés sur des plantes adultes cultivées sous serre. Matériel et méthodes. Une organogenèse a été induite à partir d'explants d'entre-noeuds sur des milieux de culture DBA3 additionnés de BAP à (0,0, 1,0, 2,0 et 3,0) mg·L^-1, combinée à de l'ANA à 0,5 mg·L^-1, et à partir d'explants de disques foliaires sur milieux de culture avec BAP (0,0, 0,5, 1,0, 1,5 et 2.0) mg·L^-1, combinée ou pas avec de l'ANA (0,5 mg·L^-1). Résultats et discussion. Les concentrations à (1,0, 2,0 ou 3.0) mg·L^-1 de BAP combinée à 0.5 mg·L^-1 d'ANA ont induit une organogenèse in vitro pour les explants d'entre-noeuds d'oranger 'Bahia'. L'utilisation des explants de disques foliaires n'a pas été efficace pour induire une organogenèse adventice.

Key words: Brazil / Citrus sinensis / micropropagation / in vitro regeneration / explants / culture media / organogenesis

Mots clés : Brésil / Citrus sinensis / micropropagation / régénération in vitro / explant / milieu de culture / organogenèse

Corresponding author: Francisco de Assis Alves Mourão Filho famourao@esalq.usp.br

© CIRAD, EDP Sciences 2006

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