EDP Sciences Journals List
Issue Fruits
Volume 59, Number 3, May-June 2004
Page(s) 181 - 190
DOI 10.1051/fruits:2004017

Fruits 59 (2004) 181-190
DOI: 10.1051/fruits:2004017

Allozymic variability in Spanish populations of Ceratitis capitata

Carmen Callejas and M. Dolores Ochando

Dpto. de Genética, Fac. CC. Biológicas, Universidad Complutense, 28040 Madrid, Spain

(Received 29 July 2003 Accepted 6 February 2004)

Abstract - Introduction. The Tephritidae Ceratitis capitata is one of the most important agricultural pests in the world. Eradication programmes need as much genetic information as possible to be efficient. It is vital to know the genetic variation that exists in the areas thought to have been important in the expansion of the species, such as the Iberian Peninsula. The aim of this study was to determine the genetic structure of Spanish C. capitata populations and the relationships between them. Materials and methods. We studied the genetic variability of populations of C. capitata (eight wild samples and one laboratory strain) through the use of the horizontal starch gel electrophoresis technique and the assay of fifteen enzyme-coding loci chosen at random. Results. The quantity of variability detected in this polyphagous species was not high. Of the 15 loci studied, only four were clearly polymorphic. No significant differences were found in any comparison of populations collected from different hosts. Discussion. The distribution patterns of this variation seem to be the result of gene flow and selection in the form of agricultural practices.

Résumé - Introduction. C. capitata, Tephritidae, est l'un des principaux ravageurs des cultures. Pour l'éradiquer de façon efficace, il est nécessaire de disposer autant que faire se peut d'informations génétiques. Pour cela, il est essentiel de connaître la variation génétique existant dans les zones qui ont été importantes pour l'expansion de l'espèce, comme l'est la péninsule ibérienne. Le but de notre étude a été de déterminer la structure génétique de certaines populations de C. capitata espagnoles et les relations existant entre elles. Matériel et méthodes. Nous avons étudié la variabilité génétique de neuf populations de C. capitata (huit échantillons sauvages prélevés soit sur figues, soit sur pêches, et une souche de laboratoire) par électrophorèse horizontale sur gel d'amidon et analyse de quinze loci d'enzymes de codage choisis au hasard. Résultats. La variabilité détectée dans cette espèce polyphage a été faible. Parmi les 15 loci étudiés, seuls quatre se sont révélés clairement polymorphes. Aucune différence significative n'a été trouvée parmi les populations de C. capitata prélevés sur les différentes plantes hôtes. Discussion. Le type de distribution de cette variation semble le résultat de flux de gènes et de sélection imputables à des pratiques agricoles.


Key words: Spain / Ceratitis capitata / electrophoresis / genetic variation

Mots clés : Espagne / Ceratitis capitata / électrophorèse / variation génétique

Correspondence and reprints: M. Dolores Ochando dochando@bio.ucm.es

© CIRAD, EDP Sciences 2004


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