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Volume 63, Number 1, January-February 2008
Page(s) 53 - 56
DOI 10.1051/fruits:2007046
Published online 24 January 2008

Fruits 63 (2008) 53-56
DOI: 10.1051/fruits:2007046

A laboratory method to evaluate Pseudocercospora musae's sensitivity to fungicides

Luc de Lapeyre de Bellaire1 and Jean-Michel Risède2

1  CIRAD, UPR Systèmes bananes et ananas, CARBAP, BP 832, Douala, Cameroon
2  CIRAD, UPR Systèmes bananes et ananas, Station de Neufchâteau Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe jean-michel.risede@cirad.fr

(Published online: 24 January 2008)

Abstract - Introduction. This protocol aims at detecting and evaluating sensitivity shifts in Pseudocercospora musae (teleomorph: Mycosphaerella musicola) populations towards fungicides that are currently sprayed to control Sigatoka disease of banana. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. Necessary laboratory materials, and details of the seven steps of the protocol achieved during four days of experiments are described. Results. The protocol results in the observation of conidial germination of P. musae (pattern or germ tube elongation) according to the sensitivity to fungicides.


Résumé - Introduction. Le protocole vise à détecter et évaluer des variations de sensibilité dans des populations de Pseudocercospora musae (téléomorphe : Mycosphaerella musicola) vis-à-vis de fongicides qui sont utilisés pour contrôler la maladie de Sigatoka chez le bananier. Le principe, les principaux avantages, le matériel végétal de départ, le temps nécessaire et les résultats attendus de la méthode sont présentés. Matériel et méthodes. Le matériel de laboratoire nécessaire et le détail des sept étapes du protocole réalisées sur quatre jours d'expérimentation sont décrits. Résultats. Le protocole aboutit à l'observation de la germination de conidies (aspect ou croissance du tube germinatif) de P. musae selon la sensibilité aux fongicides.


Key words: France (Guadeloupe) / Musa sp. / disease control / methods / laboratory equipment / pesticide resistance / Pseudocercospora musae

Mots clés : France (Guadeloupe) / Musa sp. / contrôle de maladies / méthode / matériel de laboratoire / Pseudocercospora musae / résistance aux pesticides

Corresponding author: Luc de Lapeyre de Bellaire luc.de_lapeyre@cirad.fr

© CIRAD, EDP Sciences 2008


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